Description
The Glicentin ELISA Assay Kit is a solid phase two-site enzyme immunoassay. It is based on the direct sandwich technique in which two monoclonal antibodies are directed against separate antigenic determinants on the glicentin molecule. During incubation glicentin in the sample reacts with anti-human glicentin antibodies bound to microtitration well (clone C7H9G). After washing, which removes non-reactive plasma components, a peroxidase conjugated anti-glicentin antibody (clone RG-OC5) recognizes the glicentin bound to the solid phase. After a second incubation and a simple washing step that removes unbound enzyme labeled antibody, the bound conjugate is detected by reaction with 3,3’, 5,5’-tetramethylbenzidine (TMB). The reaction is stopped by adding acid to give a colorimetric endpoint, then read spectrophotometrically